Carbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponins

It is a fundamental, underlying principle of all spectroscopy that the more absorbing species that exist in a molecule, the greater the observed signal. The standard proton is almost 100% abundant, whereas the spin active carbon nucleus is approx. 1% abundant (the remaining 99% are non-spin active isotopes). To a first approximation proton NMR spectroscopy should be much more sensitive than carbon NMR spectroscopy, and indeed it is. A proton NMR spectrum can be acquired in about 5 minutes with good signal to noise; carbon NMR spectra typically take a couple of hours (or overnight) and the signal to noise is much poorer. The proton nucleus is intrinsically much more sensitive than this in NMR experiments.

Carbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponins

carbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponins

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carbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponinscarbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponinscarbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponinscarbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponinscarbon 13 nmr spectroscopy of steroidal sapogenins and steroidal saponins